Researchers improve plant prime editing efficiency with optimized pegRNA designs

Precision genome editing enables the precise modification of DNA in living cells, thus enabling a breadth of opportunities for plant breeding. Prime editors, developed by Prof. David R. Liu and his colleagues, permit the installation of desired edits in a programmable target site. They are composed of an engineered Cas9 nickase (H840A)-reverse transcriptase (RT) fusion protein and a prime editing guide RNA (pegRNA).

Published on Thu, 25 Mar 2021 13:04:58 EDT and sponsored by power automate array contains item